Comparison of exposed amino acid residues of discrete anti-genic determinants of mouse and human LDHC/sub 4/ reveals significant differences. Furthermore, the lower degree of homology between mouse and human LDHC/submore » 4/ and the appearance of this isozyme late in evolution suggests a higher rate of mutation in the mammalian LDHC genes than in the LDHA and -B genes. Subunit homologies are consistent with the conclusion that the LDHC gene arose by at least two independent duplication events. From the deduced amino acid sequence, human LDHC/sub 4/ is as different from rodent LDHC/sub 4/ (73% homology) as it is from human LDHA/sub 4/ (76% homology) and porcine LDHB/sub 4/ (68% homology).
The sequence and structure of human testis-specific L-lactate dehydrogenase (LDHC/sub 4/, LDHX (L)-lactate:NAD/sup +/ oxidoreductase, EC 1.1.1.27) has been derived from analysis of a complementary DNA ( cDNA) clone comprising the complete protein coding region of the enzyme. These data imply a broader distribution of methanopterin-linked functions in the microbial world than previously known.« lessĮpitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence Instead, they separate from all known sequences on phylogenetic trees, pointing towards their affiliation with a novel microbial phylum. The sequences representative of the major phylotypes do not appear to belong to anymore » known microbial group capable of methanopterin-linked C 1 transfer. We conclude that more than 10 phylotypes possessing genes of interest are present in this environment, and a few of these are relatively abundant species.
In this paper we analyze the Sargasso Sea metagenome in terms of the presence of methanopterin-linked C 1 transfer genes that are signature for methylotrophy. The recently generated database of microbial genes from anoligotrophic environment populated by a calculated 1,800 of major phylotypes (the Sargasso Sea metagenome) presents a great source for expanding local databases of genes indicative of a specific function. Fishing for biodiversity: Novel methanopterin-linked C 1 transfergenes deduced from the Sargasso Sea metagenomeĭOE Office of Scientific and Technical Information (OSTI.GOV)
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